Translational and Molecular Medicine Group, Medical Research Center, Medicine Faculty, University of Antioquia.

Experience

- Several topics in Dengue Virus Infections: isolation, amplification and virus tritation; phylogenetics, Growth dynamics for evaluate antiviral agents; cell biology of virus infections with confocal live cell imaging.
- Overexpression genes in eukaryotic cells; generation of estable cell lines.
- Molecular cloning of genes and viral genomes for obtain infectious clones, replicons and viral vectors (in cooperation with Dr. Fernando Almazan)
- Isolation of viral and cellular small non-coding RNAs for computational studies (in cooperation with Dr. Clara I. Bermudez-Santana)
- Detection and quantification of cytokines, cheekiness and growth factors in serum of patients infected with dengue (in cooperation with Dr. Jhon Carlos Castaño-Osorio)
- Viral Ecology- Disease Ecology (in cooperation with Dr. Richard Hoyos-López): Field works with mosquitoes and other vectors of arbovirus (Alphavirus, Bunyavirus, Orthobunyavirus and Flavivirus); identification by mean BarCODE (mitochondrial gene), isolation and amplification of viral RNAs for identification of viral species with sequences of amplicons; identification of food source for female mosquitos (using PCRs from DNA isolated from mosquitoes); quantification of minimal infection rates.

Research interests

1. Richard Hoyos-López. BSc., MSc., Cand. PhD.
University of Sinú
Research Group in Tropical Diseases and Bacterial Resistance
rhoyoslopez@gmail.com

2. Leonardo Padilla-Sanabria. MSc., PhD.
University of Quindío
Research Group in Molecular Immunology
Director of Biomedical Research Center
leopadsa@yahoo.com

3. Jhon Carlos Castaño-Osorio. MD. PhD.
University of Quindío
Vice-chancellor of Research
Research Group in Molecular Immunology
Biomedical Research Center
jhoncarlos@uniquindio.edu.co

4. Clara I. Bermúdez-Santana
National University of Colombia
Biology Department
Theoretical and Computational RNomics Group
cibermudezs@unal.edu.co

5. Fernando Almazán
Cellular and Molecular Department
National Center of Biotechnology, CSIC.
Madrid, Spain.

Disease areas

• Dengue
• Neglected Tropical Diseases (NTDs)

Papers/reviews published in journals 1. Piedrahita, D; Castro-Alvarez, JF; Boudreau, RL; Villegas-Lanau, A; Kosik, K; Gallego-Gómez, J.C. & Cardona-Gómez, GP. 2016. ß–Secretase 1’s Targeting Reduces Hyperphosphorilated Tau, Implying Autophagy Actors in 3xTg-AD Mice. Frontiers in Cellular Neuroscience. Vol 9, art 498. 2. Roa-Linares, V; Brand, Y; Agudelo-Gomez, L; Tangarife-Castaño, V; Betancur-Galvis, L; Gallego-Gómez, J.C. & González, MA. 2016. Anti-Herpetic and Anti-Dengue Activity of Abietane Ferruginol Analogues Synthesized from (+)- Dehydroabietylamine. Journal of Medicinal Chemistry. 108: 79-88. 3. Rodríguez-Salazar, C.A., Recalde-Reyes,CP., González-MM., Padilla-Sanabria, L., Quintero-Álvarez, L., Gallego-Gómez, JC. & Jhon Carlos Castano-Osorio. Manifestaciones clínicas y hallazgos de laboratorio de una serie de casos febriles agudos con diagnóstico presuntivo de infección por el virus dengue. Quindío (Colombia). Infectio. 2015. http://dx.doi.org/10.1016/j.infect.2015.08.003. 4. Pedroza, N. C., Salazar, J. P. F., & Gómez, J. C. G. Estandarización de un protocolo para Northern blot no radioactivo usado en la detección de pequeños RNA en células Vero. Revista Colombiana de Biotecnología, 17(2), 123-129. 5. Hoyos-López, R., Suaza, JD., Tenorio, A., Uribe-Soto, S. & Gallego-Gómez, J.C. Molecular detection of Eastern Equine Encephalitis virus in mosquitoes from La Pintada (Antioquia). Revista MVZ Córdoba 20(3):4800-4806, 2015. ISSN: 0122-0268. 6. Hoyos-Lopez R, Rúa-Uribe G, Uribe S, Gallego-Gómez, J.C. Novel Genotype IV SLEV in Colombia. Mem. Inst. Oswaldo Cruz [online]. ahead of print, pp. 00-00. Epub 21-Ago-2015. ISSN 1678-8060. 7. Hoyos-López, R., Soto, S. U., & Gallego-Gómez, J. C. (2015). Evolutionary relationships of West Nile virus detected in mosquitoes from a migratory bird zone of Colombian Caribbean. Virology journal, 12(1), 80. 8. Ospina-Bedoya, M; Campillo-Pedroza, N; Franco-Salazar, JP. & Gallego-Gómez, J.C. (2014). Computational identification of Dengue virus microRNA-like structures and their cellular targets. Bioinformatics and Biology Insights. Vol. 8: 169-176. 9. Martínez-Gutiérrez, M., Correa-Londoño, L. A., Castellanos, J. E., Gallego-Gómez, J. C., & Osorio, J. E. (2014). Lovastatin Delays Infection and Increases Survival Rates in AG129 Mice Infected with Dengue Virus Serotype 2. PLOS ONE, 9(2), e87412. 10. Usme-Ciro, J., Lopera-Madrid, J., Enjuanes, L., Almazán, F. & Gallego-Gómez, J.C. (2014). "Development of a novel DNA-launched dengue virus type 2 clone under control of the cytomegalovirus promoter capable of producing infectious particles”. Virus Research. 180: 12-22. 11. Padilla-S, L., Rodríguez, A., Gonzales, MM., Gallego-G, JC., & Castaño-O, JC. (2014). Inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro. Archives of Virology. 159(3): 573-9. 12. Puerta-Arias, JD., Usme-Ciro, J. & Gallego-Gómez, J.C. (2013). Implementación de un Control Interno en la Detección Molecular de las Principales Especies de Micoplasma Contaminantes de Cultivos Celulares. Salud Uninorte, 29(2), 160-173. 13. Usme-Ciro, J., Campillo-Pedroza, N., Almazán, F. & Gallego-Gómez, J.C. Cytoplasmic RNA viruses as potential vehicles for delivery of therapeutic small RNAs. Virology Journal 2013, 10:185. doi:10.1186/1743-422X-10-185. 14. Usme-Ciro, J., Gómez-Castañeda, A. & Gallego-Gómez, J.C. Detección Molecular y Tipificación Del Virus Dengue por RT-PCR y PCR Semi-anidada usando Oligonucleótidos Mejorados. Salud Uninorte. Barranquilla (Col.) 2012; 28 (1): 1-15. 15. Mendez JA, Usme-Ciro JA, Domingo C, Rey GJ, Sanchez JA, Tenorio A, and Gallego-Gomez JC. Phylogenetic reconstruction of dengue virus type 2 in Colombia. Virology Journal 2012, 9:64. DOI:10.1186/1743-422X-9-64. 16. López-Tobón, A., Castro- Álvarez, JF., Piedrahita, D., Boudreau, RL , Gallego-Gómez, JC. & GP Cardona-Gómez. Silencing of CDK5 as potential therapy for Alzheimer ’ s disease. Reviews in the Neurosciences. 2011; 22 (2): 143-52. ISSN: 0334-1763. 17. Martínez-Gutiérrez M, Castellanos J, Gallego-Gómez JC. Statins reduce dengue virus production via decreased virion assembly. Intervirology 2011, 54(4): 11. June. ISSN: 0300-5526. 18. Mendez JA, Usme-Ciro JA, Domingo C, Rey GJ, Sanchez JA, Tenorio A, Gallego-Gomez JC. 2010. Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia. Virol J. Sep 14; 7(1): 226. ISSN: 1743-422X. 19. Piedrahita, D; Hernández, I; López-Tobón, A; Fedorov, D; Obara, B; Manjunath, BS; Boudreau, R; Davidson, B; LaFerla, F; Gallego-Gomez, JC; Kosik, K. & GP Cardona-Gomez. 2010. "Silencing of CDK5 Reduces Neurofibrillary Tangles in Transgenic Alzheimer's Mice". The Journal of Neuroscience 30: 13966-13976. ISSN 0270-6474. 20. Usme-Ciro, J., Méndez, J., Rey, G., Domingo, C., Tenorio, A. &. Gallego-Gómez, J.C. 2008. Simultaneous circulation of genotypes I and III of dengue virus 3 in Colombia. Virology Journal, Sep 2;5(1):101. ISSN: 1743-422X. Chapter books published 21. Cardona-Gomez, jc., LF. Ariza-Jimenez & Gallego-Gómez, J.C. (2016). A Proposal for a Machine Learning Classifier for Viral Infection in Living Cells Based on Mitochondrial Distribution, Cell Biology - New Insights, Prof. Stevo Najman (Ed.), ISBN: 978-953-51-2242-5, InTech, DOI: 10.5772/61293. Available from: http://www.intechopen.com/books/cell-biology-new-insights/a-proposal-for-a-machine-learning-classifier-for-viral-infection-in-living-cells-based-on-mitochondr 22. Orozco-García, E. Trujillo-Correa, A., & Gallego-Gómez, J.C. (2016). Cell Biology of Virus Infection. The Role of Cytoskeletal Dynamics Integrity in the Effectiveness of Dengue Virus Infection, Cell Biology - New Insights, Prof. Stevo Najman (Ed.), ISBN: 978-953-51-2242-5, InTech, DOI: 10.5772/61704. Available from: http://www.intechopen.com/books/cell-biology-new-insights/cell-biology-of-virus-infection-the-role-of-cytoskeletal-dynamics-integrity-in-the-effectiveness-of- 23. Orozco-García, E. & Gallego-Gómez, J.C. (2016). Autophagy and Lipid Metabolism – A Cellular Platform where Molecular and Metabolic Pathways Converge to Explain Dengue Viral Infection, Cell Biology - New Insights, Prof. Stevo Najman (Ed.), ISBN: 978-953-51-2242-5, InTech, DOI: 10.5772/61305. Available from: http://www.intechopen.com/books/cell-biology-new-insights/autophagy-and-lipid-metabolism-a-cellular-platform-where-molecular-and-metabolic-pathways-converge-t 24. Usme-Ciro, J., Hoyos-López, R. Gallego-Gómez, J.C. 2012. "Viral Evolutionary Ecology- Conceptual Basis of a New Scientific Approach for Understanding Viral Emergence”. Libro: Epidemiology. Current Perspectives on Research and Practice", ISBN 979-953-307-498-5, editorial: InTech Open Access Publisher. http://www.intechopen.com/articles/show/title/viral-evolutionary-ecology-conceptual-basis-of-a-new-scientific-approach-for-understanding-viral-eme 25. Gallego-Gómez, J.C. 2013. “Darwin y Algunas Reflexiones en Filosofía, Sociología e Historia de la biología”. pp: 325-342. Libro: Filosofía de la Ciencia. Problemas Contemporáneos. Compiladores: Germán Guerrero-Pino y Luz Marina Duque. Santiago de Cali – Programa Editorial Universidad del Valle. ISBN 978-958-765-166-9.

Other information

In my group also we are interested in topics o ST&S related with the factors for health-disease process.